Large dense-core vesicle exocytosis in pancreatic β-cells monitored by capacitance measurements
T Kanno, X Ma, S Barg, L Eliasson, J Galvanovskis… - Methods, 2004 - Elsevier
Methods, 2004•Elsevier
This article discusses the currently used methodologies for monitoring exocytosis as
changes in cell capacitance. Details are given on composition of solutions, experimental
protocols, and how the observed responses can be interpreted physiologically. The
concepts are illustrated by examples from our own work on insulin-releasing pancreatic β-
cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine
cells in intact pancreatic islets, where the cells are electrically coupled to each other.
changes in cell capacitance. Details are given on composition of solutions, experimental
protocols, and how the observed responses can be interpreted physiologically. The
concepts are illustrated by examples from our own work on insulin-releasing pancreatic β-
cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine
cells in intact pancreatic islets, where the cells are electrically coupled to each other.
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic β-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.
Elsevier